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abbexa abx370872?說(shuō)明書(shū)

 更新時(shí)間:2022-06-24 點(diǎn)擊量:493

Abbexa提供和經(jīng)銷生命科學(xué),制藥和生物技術(shù)方面的產(chǎn)品。公司位于英國(guó)劍橋,產(chǎn)品類 別包括一抗、二抗、同型對(duì)照、蛋白質(zhì)、酶聯(lián)免疫 (ELISA)試劑盒和酶等生物試劑。公司與世界不同實(shí)驗(yàn)室合作,研發(fā)與生物醫(yī)學(xué)研究市場(chǎng)相關(guān)的高質(zhì)量經(jīng)過(guò)檢驗(yàn)并且價(jià)格合理的產(chǎn)品,以滿足客戶的需要。近8000種一抗,包括少見(jiàn)的抗體如鮭魚(yú)朊病毒單抗、霍亂內(nèi)毒素EtxB單抗、糖基化載脂蛋白-B單抗、子宮內(nèi)膜抗原單抗等,多種信號(hào)通路研究相關(guān)的抗體,很多不同位點(diǎn)磷酸化蛋白的抗體等。


abbexa abx370872說(shuō)明書(shū)



1,25-Dihydroxyvitamin D3 (Calcitriol) Antibody Pair

貨號(hào): abx370872



用于競(jìng)爭(zhēng)性 ELISA 檢測(cè)開(kāi)發(fā)的骨化3醇抗體對(duì)。該抗體對(duì)包含:


零件5 × 96 次測(cè)試10 × 96 次測(cè)試
捕獲抗體200微克400微克
生物素共軛競(jìng)爭(zhēng)者50微克100微克
標(biāo)準(zhǔn)2微克10微克


請(qǐng)注意,不同批次之間的數(shù)量和濃度可能會(huì)有所不同。

建議將此抗體對(duì)與abx098959 抗體對(duì)支持試劑盒(競(jìng)爭(zhēng)方法)一起使用。

目標(biāo)1,25-二羥基維生素(骨化3醇)
反應(yīng)性人類
測(cè)試應(yīng)用酶聯(lián)免疫吸附試驗(yàn)
推薦稀釋用包被緩沖液將 Capture Antibody 稀釋 125 倍。
用生物素共軛競(jìng)爭(zhēng)對(duì)手稀釋劑將生物素共軛競(jìng)爭(zhēng)對(duì)手稀釋 200 倍。


最佳稀釋度/濃度應(yīng)由最終用戶確定。
形式液體(捕獲抗體和生物素偶聯(lián)的競(jìng)爭(zhēng)對(duì)手)
重組用標(biāo)準(zhǔn)稀釋劑重新配制標(biāo)準(zhǔn)。體積和標(biāo)準(zhǔn)濃度應(yīng)由最終用戶確定。
貯存分裝并儲(chǔ)存在 -20°C。避免重復(fù)凍融循環(huán)。
緩沖Capture Antibody 和 Biotin-Conjugated Competor 均含有 0.1% 疊氮化納。
測(cè)試范圍123.5 皮克/毫升 - 10000 皮克/毫升
標(biāo)準(zhǔn)格式凍干
檢測(cè)類型競(jìng)爭(zhēng)的
捕獲抗體宿主兔子
捕獲抗體克隆多克隆
捕獲抗體偶聯(lián)未結(jié)合的
檢測(cè)抗體偶聯(lián)生物素
專注捕獲抗體:0.5 mg/ml
Biotin-Conjugated 競(jìng)爭(zhēng)對(duì)手:0.2 mg/ml
使用說(shuō)明將所有組件置于室溫 (18-25°C) 并在使用前短暫旋轉(zhuǎn)或離心小瓶。應(yīng)立即準(zhǔn)備和使用工作溶液。

推薦程序:

  1. 使用包被緩沖液將 Capture Antibody 稀釋至工作濃度。立即用稀釋的 Capture Antibody(每孔 100 µl)包被 96 孔板。密封板并在 4 °C 下孵育過(guò)夜或在 37 °C 下孵育 2 小時(shí)

  2. 吸出孔并用洗滌緩沖液(每孔 350 µl)洗滌,浸泡 1-2 分鐘。將板倒置并輕敲到吸水紙上,以除去液體。

  3. 用封閉緩沖液(每孔 200 µl)在 37 °C 下封閉板 1.5 小時(shí)。

  4. 重復(fù)步驟 2 中的抽吸/清洗過(guò)程。

  5. 將 50 µl 標(biāo)準(zhǔn)品或樣品添加到適當(dāng)?shù)目字?,然后添?50 µl 工作的 Biotin-Conjugated Competor。蓋上封板劑并在 37 °C 下孵育 1 小時(shí)。

  6. 重復(fù)步驟 2 中的抽吸/清洗過(guò)程。

  7. 加入適當(dāng)稀釋的鏈霉親和素 HRP(每孔 100 µl)。用新的封板劑蓋住盤子,在 37°C 下孵育 30 分鐘。

  8. 重復(fù)步驟 2 中的抽吸/清洗過(guò)程,共 5 次。

  9. 添加底物溶液(每孔 90 µl)。用新的封板機(jī)蓋住盤子,在 37°C 下孵育 10-20 分鐘。將盤子放在黑暗中,避免暴露在光線下。

  10. 添加終止液(每孔 50 µl)。輕敲板的側(cè)面以確保*混合。

  11. 使用設(shè)置為 450 nm 的酶標(biāo)儀立即測(cè)量吸光度。

可用性5-20 個(gè)工作日內(nèi)發(fā)貨。
筆記本產(chǎn)品僅供研究使用。






ModelCategoryName

abx000001Primary AntibodiesHistone H3K27AC Antibody

abx000002Primary AntibodiesHistone H3K4me1 Antibody

abx000003Primary AntibodiesHistone H3K4me2 Antibody

abx000004Primary AntibodiesHistone H3K4me3 Antibody

abx000005Primary AntibodiesHistone H3K9me1 Antibody

abx000006Primary AntibodiesHistone H3K9me2 Antibody

abx000007Primary AntibodiesHistone H3K9me3 Antibody

abx000008Primary AntibodiesHistone H3K27me1 Antibody

abx000009Primary AntibodiesHistone H3K27me2 Antibody

abx000010Primary AntibodiesHistone H3K27me3 Antibody

abx000011Primary AntibodiesHistone H3K36me1 Antibody

abx000012Primary AntibodiesHistone H3K36me2 Antibody

abx000013Primary AntibodiesHistone H3K36me3 Antibody

abx000014Primary AntibodiesHistone H3K79me1 Antibody

abx000015Primary AntibodiesHistone H3K79me2 Antibody

abx000016Primary AntibodiesHistone H3K79me3 Antibody

abx000017Primary AntibodiesHistone H4K20me1 Antibody

abx000018Primary AntibodiesHistone H4K20me2 Antibody

abx000019Primary AntibodiesHistone H4K20me3 Antibody

abx000020Primary AntibodiesHistone H3R2me2s Antibody

abx000021Primary AntibodiesHistone H3R8me2s Antibody

abx000022Primary AntibodiesHistone H3R26me2a Antibody

abx000023Primary AntibodiesHistone H4R3me2a Antibody

abx000024Primary AntibodiesHistone H3R17me2a Antibody

abx000025Primary AntibodiesHistone H3R17me1 Antibody

abx000026Primary AntibodiesHistone H3R17me2s Antibody

abx000027Primary AntibodiesHistone H3R26me2s Antibody

abx000028Primary AntibodiesHistone H3R2me1 Antibody

abx000029Primary AntibodiesHistone H3R2me2a Antibody

abx000030Primary AntibodiesHistone H3R8me1 Antibody

abx000031Primary AntibodiesHistone H3R8me2a Antibody

abx000033Primary AntibodiesHistone H4R3me2s Antibody

abx000034Primary AntibodiesHistone H3R26me1 Antibody

abx000035Primary AntibodiesDimethyl-Histone H3-K14 Antibody

abx000036Primary AntibodiesTrimethyl-Histone H3-K14 Antibody

abx000037Primary AntibodiesPan Dimethyl-lysine Antibody

abx000038Primary AntibodiesHistone H3(mono+di+methyl K79) Antibody

abx000039Primary AntibodiesTumor Necrosis Factor Receptor Superfamily Member 1A / CD120a (TNFRSF1A) Antibody

abx000040Primary AntibodiesHepatocyte Growth Factor Receptor (MET) Antibody

abx000042Primary AntibodiesTransforming Growth Factor Beta 1 (TGFB1) Antibody

abx000043Primary AntibodiesMethylenetetrahydrofolate Reductase (MTHFR) Antibody

abx000044Primary AntibodiesAlbumin (ALB) Antibody

abx000047Primary AntibodiesChemokine C-X-C-Motif Receptor 4 (CXCR4) Antibody

abx000061Primary AntibodiesApolipoprotein A-V (APOA5) Antibody

abx000063Primary AntibodiesMyeloid/lymphoid Or Mixed-Lineage Leukemia (MLL) Antibody

abx000064Primary AntibodiesBromodeoxyuridine (BrdU) Antibody

abx000067Primary AntibodiesHeat Shock Protein 70 (HSP70) Antibody

abx000082Primary AntibodiesCadherin-1 / E-Cadherin (CDH1) Antibody

abx000083Primary AntibodiesCrystallin Beta B2 (CRYBB2) Antibody

abx000084Primary AntibodiesCaudal Type Homeobox 1 (CDX1) Antibody

abx000091Primary AntibodiesABL1 (pY245) Antibody

abx000093Primary AntibodiesABL1 (pY204) Antibody

abx000094Primary AntibodiesRAC-Alpha Serine/threonine-Protein Kinase Phospho-Thr450 (AKT1 pT450) Antibody

abx000095Primary AntibodiesRAC-Beta Serine/Threonine-Protein Kinase Phospho-Ser474 (AKT2 pS474) Antibody

abx000096Primary AntibodiesAPP (pT743) Antibody

abx000097Primary AntibodiesFAS (pY291) Antibody

abx000098Primary AntibodiesATM (pS1981) Antibody

abx000099Primary AntibodiesBad (pS118) Antibody

abx000100Primary AntibodiesBad (pS75) Antibody

abx000101Primary AntibodiesBad (pS99) Antibody

abx000102Primary AntibodiesG1/S-Specific Cyclin-E1 Phospho-Thr395 (CCNE1 pT395) Antibody

abx000104Primary AntibodiesCDK1 (pT14) Antibody

abx000105Primary AntibodiesCDK1 (pY15) Antibody

abx000106Primary AntibodiesCHEK1 (pS280) Antibody

abx000107Primary AntibodiesCHEK1 (pS317) Antibody

abx000108Primary AntibodiesCREB (pS133) Antibody

abx000109Primary AntibodiesATF2 (pT53) Antibody

abx000110Primary AntibodiesDnmt1 (phospho-pS714) Antibody

abx000111Primary AntibodiesDnmt1 (phospho-pY399) Antibody

abx000112Primary AntibodiesEGFR (pY869) Antibody

abx000113Primary AntibodiesEGFR (pY998) Antibody

abx000114Primary AntibodiesEGFR (pT693) Antibody

abx000115Primary AntibodiesEGFR (pY1016) Antibody

abx000116Primary AntibodiesEGFR (pY1172) Antibody

abx000117Primary AntibodiesEIF4EBP1 (pS65) Antibody

abx000118Primary AntibodiesELK1 (pS383) Antibody

abx000119Primary AntibodiesHER4 (pY1284) Antibody

abx000120Primary AntibodiesFGF Receptor 1 (pY766) Antibody

abx000121Primary AntibodiesFGF Receptor 1 (pY653) Antibody

abx000122Primary AntibodiesFGF Receptor 1 (pY654) Antibody

abx000123Primary AntibodiesProto-Oncogene C-Fos (pT232) (FOS) Antibody

abx000124Primary AntibodiesGSK3 (pS9) Antibody

abx000125Primary AntibodiesHSPB1 (pS15) Antibody

abx000126Primary AntibodiesHSPB1 (pS82) Antibody

abx000127Primary AntibodiesInsulin Receptor beta (pY1361) Antibody

abx000128Primary AntibodiesINSR (pY1146) / IGF1R (pY1131) Antibody

abx000129Primary AntibodiesINSR (pY1150) / IGF1R (pY1135) Antibody

abx000130Primary AntibodiesINSR (pY1151) / IGF1R (pY1136) Antibody

abx000131Primary AntibodiesInsulin Receptor beta (pY1355) Antibody

abx000132Primary Antibodiesc-Jun (pS73) Antibody

abx000133Primary Antibodiesc-Jun (pS63) Antibody

abx000134Primary Antibodiesc-Jun (pT239) Antibody

abx000135Primary Antibodiesc-Jun (pS243) Antibody

abx000136Primary AntibodiesERK3 (pS189) Antibody

abx000137Primary AntibodiesMAPK14 (pY322) Antibody

abx000138Primary AntibodiesMAPK14 (pT180) Antibody

abx000139Primary AntibodiesMAPK14 (pY182) Antibody

abx000140Primary AntibodiesMAP2K1 (pS217) Antibody

abx000141Primary AntibodiesMAP2K1 (pS297) Antibody

abx000142Primary AntibodiesMAP2K1 (phospho-pS221) Antibody

abx000143Primary AntibodiesMAP2K3 (pS189) Antibody

abx000144Primary AntibodiesSignal Transducer and Activator of Transcription 3 Phospho-Tyr705 (STAT3 pY705) Antibody

abx000145Primary AntibodiesMAX (pS11) Antibody

abx000146Primary AntibodiesMDM2 (pS166) Antibody

abx000147Primary AntibodiesMDM2 (pS260) Antibody

abx000148Primary AntibodiesMEF2C (pS396) Antibody

abx000149Primary AntibodiesMET (pY1003) Antibody

abx000150Primary AntibodiesMET (pY1349) Antibody

abx000151Primary AntibodiesMET (pY1234) Antibody

abx000152Primary AntibodiesMET (pY1235) Antibody

abx000153Primary AntibodiesMYC (pT58) Antibody

abx000154Primary AntibodiesMAP2K6 (pS207) Antibody

abx000155Primary AntibodiesMYC (pS62) Antibody

abx000156Primary Antibodiesp53 Phospho-Ser15 (p53 pS15) Antibody

abx000157Primary AntibodiesTP53 (pS9) Antibody

abx000158Primary AntibodiesRAC1 (pS71) Antibody

abx000159Primary AntibodiesRAF1 (pS621) Antibody

abx000160Primary AntibodiesRetinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody

abx000161Primary AntibodiesRetinoblastoma Protein 1 Phospho-Ser811 (RB1 pS811) Antibody

abx000162Primary AntibodiesSMC1A (pS957) Antibody

abx000163Primary AntibodiesHIST1H3B (pT12) Antibody

abx000164Primary AntibodiesMTOR (pS2448) Antibody

abx000165Primary AntibodiesHIST1H3B (pS29) Antibody

abx000166Primary AntibodiesRAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) Antibody

abx000167Primary AntibodiesHistone H2A.x (pS139) Antibody

abx000168Primary AntibodiesBtk (pY223) Antibody

abx000170Primary AntibodiesAMPK alpha (pT172) Antibody